InSight®X3+ and InSight®X3

WIDELY TUNABLE ULTRAFAST LASERS FOR MULTIPHOTON IMAGING

  • High power across entire tuning range with the industry’s highest power at
         the multiphoton imaging wavelengths between 900 and 1300 nm
  • Field proven with the largest installed base in the market,
         hundreds of peer-reviewed publications and demonstrated high reliability
  • Broad tuning range (680–1300 nm) for maximum flexibility
  • Dual output at 1045 nm with optional pre-compensation for simultaneous two color excitation

The InSight® X3+ and InSight X3 are built on Spectra-Physics’ industry leading InSight platform designed for maximum performance and reliability. The InSight X3+ delivers 50% greater power across the tuning range for deeper imaging and more complex experiments. The InSight X3+ and InSight X3 lasers are built on the proven InSight platform with hundreds of peer-reviewed publications, the largest installed base in the market, and demonstrated high reliability. The InSight platform is specifically designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences.

Based on patented technology1, InSight features a broad 680 nm to 1300 nm continuous, gap free, tuning curve from a single beam with nearly double the tuning range of legacy Ti:Sapphire lasers. The InSight is designed with the peak of the tuning curve centered in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ additionally provides the highest powers at the longest wavelengths of 1280–1300 nm for excitation of far red fluorophores such as Alexa-680.

With Spectra-Physics’ integrated and patented DeepSee, the industry standard dispersion pre-compensation module, the short femtosecond pulses are optimally delivered through a microscope to the sample providing maximum fluorescence and penetration depth. The InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning.

When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable easy simultaneous imaging of various fluorescence proteins, genetically encoded calcium indicators, second and third harmonic generation, and advanced imaging techniques such as CARS and SRS. For these advanced techniques the InSight can be combined with the Newport Timing and Recombination Unit (TRU), or the Spectral Focusing Timing and Recombination Unit (SF-TRU).

1. Patents

Applications

Multiphoton microscopy
Two-Photon Excited Fluorescence (TPEF)
Multimodal imaging
-Coherent anti-stokes Raman Spectroscopy (CARS)
-Stimulated Raman spectroscopy (SRS)
-Second harmonic generation (SHG)
-Third harmonic generation (THG)
Optogenetics
Non-linear spectroscopy
Time-resolved photoluminescence
Optical computed tomography
Terahertz imaging
Semiconductor metrology
   
 InSight X3+InSight X3  
 Main OutputDual OutputMain OutputDual Output  
Tuning Range 680 nm–1300 nm 1045 nm (fixed) 680 nm–1300 nm 1045 nm (fixed)  
Average Power2 >1.3 W at 700 nm
>2.4 W at 800 nm
>3.0 W at 900 nm
>2.7 W at 1000 nm
>2.4 W at 1100 nm
>2.1 W at 1200 nm
>1.5 W at 1300 nm
>3.5 W at 1045 nm >1.0 W at 700 nm
>1.6 W at 800 nm
>2.0 W at 900 nm
>1.8 W at 1000 nm
>1.6 W at 1100 nm
>1.4 W at 1200 nm
>1.0 W at 1300 nm
>3.5 W at 1045 nm  
Pulse Width3,6 <120 fs <170 fs <120 fs <200 fs  
Noise3,4, 6 <0.5% <0.25% <0.5% <0.5%  
Pre-compensation Dispersion Range2 680 nm: -12,000 fs2 to -40,000 fs2
800 nm: 0 fs2 to -28,000 fs2
900 nm: 0 fs2 to -17,000 fs2
1050 nm: 0 fs2 to -13,000 fs2
1300 nm: -3,000 fs2 to -11,000 fs2
"Optional
1045 nm: 15,000 fs2 fixed"
680 nm: -12,000 fs2 to -40,000 fs2
800 nm: 0 fs2 to -25,000 fs2
1050 nm: 0 fs2 to -10,000 fs2
1300 nm: -3,000 fs2 to -8,000 fs2
"Optional
1045 nm: -15,000 fs2 fixed"
 
Repetition Rate7 80 MHz ±0.5  
Spatial Mode3 TEM00, M2 <1.2  
Beam Diameter (1/e²)3 1.1 ±0.2 mm  
Beam Divergence, full angle3 <1.5 mrad  
Beam Roundness3 1.0 ±0.2 mm  
Beam Astigmatism³3 <20 %  
Beam Pointing Stability9 <350 µrad  
Tuning Speed10 >50 nm/sec  
Stability5 <±1 %  
Polarization3 >500:1 horizontal  
   
 InSight X3+InSight X3  
 Main OutputDual OutputMain OutputDual Output  
Altitude (m) Up to 2000 M  
Temperature, Operating 20–25°C  
Relative Humidity, Operating Maximum 75% non-condensing up to 25°C  
Temperature, Storage 15–35°C  
Relative Humidity, Storage Maximum 65% for 15–35°C  
Cooled Water Temperature in Closed-loop Chiller 21°C typical8  
   
 InSight X3+InSight X3  
 Main OutputDual OutputMain OutputDual Output  
Power Supply 100–120 V~/200–240 V~, 50/60 Hz  
Chiller 100–200 V~, 50/60 HzC  
Total System Power Consumption <1500 W
Communication Interface RS-232, USB, PC required  
Sync Signal SMA  

1. Due to our continuous improvement program, specifications may change without notice.
2. Specifications only apply to the wavelength noted.
3. Specification applies to 900 nm (tunable) or 1045 nm (fixed), respectively.
4. Specification represents rms noise measured in a 10 Hz to 10 MHz bandwidth.
5. Percent power drift in any 2-hour period with less than ±1°C temperature change after a 1-hour warm up.
6. A sech2 pulse shape is used to determine the pulse width as measured with a Newport PulseScout® autocorrelator.
7. Tunable and fixed outputs are synchronized.
8. Avoid obstructing the air exhaust grills which will result in the recirculation of hot exhaust air.
Cooling air enters through the front panel and exits through the rear fan apertures.
9. Maximum deviation across the entire tuning range and pre-compensation dispersion range.
10. Averaged over entire tuning range.
11. InSight X3+ and InSight X3 are Class IV – High-Power Lasers, whose beams are, by definition, safety and fire hazards.
Take precautions to prevent exposure to direct and reflected beams. Diffuse as well as specular reflections can cause severe skin or eye damage.

InSight X3+ and InSight X3 Dimensions

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