Widely Tunable Ultrafast Lasers for Multiphoton Imaging
InSight X3+ | InSight X3 | ||||
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Main Output | Dual Output | Main Output | Dual Output | ||
Tuning Range | 680 nm-1300 nm | 1045 nm (fixed) | 680 nm-1300 nm | 1045 nm (fixed) | |
Average Power2 | >1.3 W at 700 nm >2.4 W at 800 nm >3.0 W at 900 nm >2.7 W at 1000 nm >2.4 W at 1100 nm >2.1 W at 1200 nm >1.5 W at 1300 nm |
>3.5 W at 1045 nm | >1.0 W at 700 nm >1.6 W at 800 nm >2.0 W at 900 nm >1.8 W at 1000 nm >1.6 W at 1100 nm >1.4 W at 1200 nm >1.0 W at 1300 nm |
>3.5 W at 1045 nm | |
Pulse Width3,6 | <120 fs | <170 fs | <120 fs | <200 fs | |
Noise3,4, 6 | <0.5% | <0.25% | <0.5% | <0.5% | |
Pre-compensation Dispersion Range2 | 680 nm: -12,000 fs2 to -40,000 fs2 800 nm: 0 fs2 to -28,000 fs2 900 nm: 0 fs2 to -17,000 fs2 1050 nm: 0 fs2 to -13,000 fs2 1300 nm: -3,000 fs2 to -11,000 fs2 |
"Optional 1045 nm: 15,000 fs2 fixed" |
680 nm: -12,000 fs2 to -40,000 fs2 800 nm: 0 fs2 to -25,000 fs2 1050 nm: 0 fs2 to -10,000 fs2 1300 nm: -3,000 fs2 to -8,000 fs2 |
"Optional 1045 nm: -15,000 fs2 fixed" |
|
Repetition Rate7 | 80 MHz ±0.5 | ||||
Spatial Mode3 | TEM00, M2 <1.2 | ||||
Beam Diameter (1/e²)3 | 1.1 ±0.2 mm | ||||
Beam Divergence, full angle3 | <1.5 mrad | ||||
Beam Roundness3 | 1.0 ±0.2 mm | ||||
Beam Astigmatism³3 | <20 % | ||||
Beam Pointing Stability9 | <350 µrad | ||||
Tuning Speed10 | >50 nm/sec | ||||
Stability5 | <±1 % | ||||
Polarization3 | >500:1 horizontal |
InSight X3+ | InSight X3 | ||||
---|---|---|---|---|---|
Main Output | Dual Output | Main Output | Dual Output | ||
Altitude (m) | Up to 2000 M | ||||
Temperature, Operating | 20-25°C | ||||
Relative Humidity, Operating | Maximum 75% non-condensing up to 25°C | ||||
Temperature, Storage | 15-35°C | ||||
Relative Humidity, Storage | Maximum 65% for 15-35°C | ||||
Cooled Water Temperature in Closed-loop Chiller | 21°C typical8 |
InSight X3+ | InSight X3 | ||||
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Main Output | Dual Output | Main Output | Dual Output | ||
Power Supply | 100-120 V~/200-240 V~, 50/60 Hz | ||||
Chiller | 100-200 V~, 50/60 HzC | ||||
Total System Power Consumption | <1500 W | ||||
Communication Interface | RS-232, USB, PC required | ||||
Sync Signal | SMA |
Footnotes:
The InSight® X3+™ and InSight® X3™ are built on the industry leading InSight platform designed for maximum performance and reliability with the largest installed base in the market. The InSight X3+ delivers 50% greater power across the tuning range for deeper imaging and more complex experiments. The InSight platform is specifically designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences.
Based on patented technology, InSight features a broad 680 nm to 1300 nm continuous, gap free, tuning curve from a single beam with nearly double the tuning range of legacy Ti:Sapphire lasers. The InSight is designed with the peak of the tuning curve centered in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ additionally provides the highest powers at the longest wavelengths of 1280–1300 nm for excitation of far red fluorophores such as Alexa-680.
With Spectra-Physics’ integrated and patented DeepSee™, the industry standard dispersion pre-compensation module, the short femtosecond pulses are optimally delivered through a microscope to the sample providing maximum fluorescence and penetration depth. The InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning.
When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable easy simultaneous imaging of various fluorescence proteins, genetically encoded calcium indicators, second and third harmonic generation, and advanced imaging techniques such as CARS and SRS.