InSight^®X3+™ and InSight^®X3™ Tunable Ultrafast Lasers

Overview
Applications
Specifications
Features
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Overview

Widely Tunable Ultrafast Lasers for Multiphoton Imaging

Field proven with the largest installed base in the market
High reliability with >950 peer-reviewed publications
Broad tuning range (680–1300 nm) for maximum flexibility
Highest peak power for maximum fluorescence
InSight X3+ A and InSight X3 A models with integrated attenuation

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Products

Applications

Specifications

Characteristics^{1, 8}

	InSight X3+		InSight X3
Output Characteristics	Main Output	Dual Output	Main Output	Dual Output
Tuning Range	680 nm-1300 nm	1045 nm (fixed)	680 nm-1300 nm	1045 nm (fixed)
Average Power²	>1.5 W at 700 nm >2.4 W at 800 nm >3.0 W at 900 nm >2.7 W at 1000 nm >2.4 W at 1100 nm >2.1 W at 1200 nm >1.5 W at 1300 nm	>3.5 W	>1.0 W at 700 nm >1.6 W at 800 nm >2.0 W at 900 nm >1.8 W at 1000 nm >1.6 W at 1100 nm >1.4 W at 1200 nm >1.0 W at 1300 nm	>3.5 W
Fixed Beam (1045 nm) Power	>3.5 W	>3.0 W	>3.5 W	>3.0 W
Pulse Width²	<120 fs	<170 fs	<120 fs	<200 fs
Noise^2,3,4	<0.5 %	<0.25 %	<0.5 %	<0.5 %
Pre-compensation Dispersion Range	680 nm: -12,000 fs² to -40,000 fs² 800 nm: 0 fs² to -28,000 fs² 900 nm: 0 fs² to -17,000 fs² 1050 nm: 0 fs² to -13,00 fs² 1300 nm: -3,000 fs² to -11,00 fs²	"Optional: -14,000 fs² fixed"	680 nm: -12,000 fs² to -40,000 fs² 800 nm: 0 fs² to -25,000 fs² 1050 nm: 0 fs² to -10,00 fs² 1300 nm: -3,000 fs² to -8,000 fs²	"Optional: -14,000 fs² fixed"
Repetition Rate	80 MHz ±0.5
Spatial Mode²	TEM₀₀, M² <1.2
Beam Diameter (1/e²)²	1.1 ±0.2 mm
Beam Divergence, full angle²	<1.5 mrad
Beam Roundness²	0.8-1.2
Beam Astigmatism²	<20 %
Beam Pointing Stability⁵	<350 µrad
Tuning Speed⁶	>50 nm/sec
Stability⁷	<±1 %
Polarization²	>500:1 horizontal

Environmental Requirements

	InSight X3+		InSight X3
Altitude	Up to 2000 m
Temperature, Operating	20-25°C
Relative Humidity, Operating	Maximum 75% non-condensing up to 25°C
Temperature, Storage	15-35°C
Relative Humidity, Storage	Maximum 65% for 15-35°C
Cooled Water Temperature in Closed-loop Chiller	21°C, Typical

Electrical Requirements

	InSight X3+		InSight X3
Power Supply	100-120 V~/200-240 V~, 50/60 Hz
Chiller	100-200 V~, 50/60 HzC
Total System Power Consumption	<1500 W
Communication Interface	RS-232, USB, PC required
Sync Signal	SMA

Footnotes:

Due to our continuous improvement program, specifications may change without notice.
Specification applies to 900 nm (tunable) output.
A sech² pulse shape is used to determine the pulse width as measured with a Newport PulseScout^® autocorrelator.
Specification represents rms noise measured in a 10 Hz to 10 MHz bandwidth.
Maximum deviation across the entire tuning range and precompensation dispersion range.
Averaged over entire tuning range.
Percent power drift in any 2-hour period with less than ±1°C temperature change after a 1-hour warm up.
InSight X3+ and InSight X3 are Class IV – High- Power Lasers, whose beams are, by definition, safety and fire hazards. Take precautions to prevent exposure to direct and reflected beams. Diffuse as well as specular reflections can cause severe skin or eye damage.

Specifications

Characteristics^{1, 8}

	InSight X3+		InSight X3
Output Characteristics	Main Output	Dual Output	Main Output	Dual Output
Tuning Range	680 nm-1300 nm	1045 nm (fixed)	680 nm-1300 nm	1045 nm (fixed)
Average Power²	>1.5 W at 700 nm >2.4 W at 800 nm >3.0 W at 900 nm >2.7 W at 1000 nm >2.4 W at 1100 nm >2.1 W at 1200 nm >1.5 W at 1300 nm	>3.5 W	>1.0 W at 700 nm >1.6 W at 800 nm >2.0 W at 900 nm >1.8 W at 1000 nm >1.6 W at 1100 nm >1.4 W at 1200 nm >1.0 W at 1300 nm	>3.5 W
Fixed Beam (1045 nm) Power	>3.5 W	>3.0 W	>3.5 W	>3.0 W
Pulse Width²	<120 fs	<170 fs	<120 fs	<200 fs
Noise^2,3,4	<0.5 %	<0.25 %	<0.5 %	<0.5 %
Pre-compensation Dispersion Range	680 nm: -12,000 fs² to -40,000 fs² 800 nm: 0 fs² to -28,000 fs² 900 nm: 0 fs² to -17,000 fs² 1050 nm: 0 fs² to -13,00 fs² 1300 nm: -3,000 fs² to -11,00 fs²	"Optional: -14,000 fs² fixed"	680 nm: -12,000 fs² to -40,000 fs² 800 nm: 0 fs² to -25,000 fs² 1050 nm: 0 fs² to -10,00 fs² 1300 nm: -3,000 fs² to -8,000 fs²	"Optional: -14,000 fs² fixed"
Repetition Rate	80 MHz ±0.5
Spatial Mode²	TEM₀₀, M² <1.2
Beam Diameter (1/e²)²	1.1 ±0.2 mm
Beam Divergence, full angle²	<1.5 mrad
Beam Roundness²	0.8-1.2
Beam Astigmatism²	<20 %
Beam Pointing Stability⁵	<350 µrad
Tuning Speed⁶	>50 nm/sec
Stability⁷	<±1 %
Polarization²	>500:1 horizontal

Environmental Requirements

	InSight X3+		InSight X3
Altitude	Up to 2000 m
Temperature, Operating	20-25°C
Relative Humidity, Operating	Maximum 75% non-condensing up to 25°C
Temperature, Storage	15-35°C
Relative Humidity, Storage	Maximum 65% for 15-35°C
Cooled Water Temperature in Closed-loop Chiller	21°C, Typical

Electrical Requirements

	InSight X3+		InSight X3
Power Supply	100-120 V~/200-240 V~, 50/60 Hz
Chiller	100-200 V~, 50/60 HzC
Total System Power Consumption	<1500 W
Communication Interface	RS-232, USB, PC required
Sync Signal	SMA

Footnotes:

Due to our continuous improvement program, specifications may change without notice.
Specification applies to 900 nm (tunable) output.
A sech² pulse shape is used to determine the pulse width as measured with a Newport PulseScout^® autocorrelator.
Specification represents rms noise measured in a 10 Hz to 10 MHz bandwidth.
Maximum deviation across the entire tuning range and precompensation dispersion range.
Averaged over entire tuning range.
Percent power drift in any 2-hour period with less than ±1°C temperature change after a 1-hour warm up.
InSight X3+ and InSight X3 are Class IV – High- Power Lasers, whose beams are, by definition, safety and fire hazards. Take precautions to prevent exposure to direct and reflected beams. Diffuse as well as specular reflections can cause severe skin or eye damage.

Features

Overview

The InSight X3+ and InSight X3 are built on Spectra-Physics’ industry leading InSight platform. Designed for maximum performance and reliability, the InSight X3+ delivers 50% high powers across the tuning range for brighter signals and increased imaging depths. The InSight X3+ and InSight X3 lasers have the largest installed base with field proven reliability. As a result, researchers have generated hundreds of peer-reviewed publications.

Ultra-Wide Tuning Range

The InSight platform is designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences. Based on patented technology1 , InSight features a broad, gap free tuning curve that spans 680 nm to 1300 nm; nearly doubling the wavelength coverage of legacy Ti:Sapphire lasers. The InSight tuning curve peaks in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ provides higher powers at the longest wavelengths between 1280–1300 nm for excitation of far red fluorophores.

Exceptional Performance

With Spectra-Physics’ integrated and patented DeepSee™ dispersion pre-compensation module, short femtosecond pulses are optimally delivered through the microscope to provide maximum fluorescence and penetration depth. The InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning. When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable simultaneous imaging of various fluorescence proteins, genetically encoded calcium indicators, second and third harmonic generation, and advanced imaging techniques such as CARS and SRS.

InSight X3+ A and InSight X3 A Models

Built on the proven InSight platform, InSight X3+ A and InSight X3 A lasers are equipped with integrated attenuation for real-time optimization of the experimental pulse energy. Each model delivers high average power, short pulses and dispersion compensation to support the most demanding experiments in neuroscience, immunology and biology.

Compatible Power Sensors

To verify and analyze the output power of your InSight X3+/X3 laser, we recommend the Ophir 3A (7Z02621) thermal power sensor and Centauri laser power meter. Additional information on this laser measurement equipment can be found on the ophiropt.com website. Other Ophir sensors and meters may also be suitable for the InSight X3+/X3, depending on application. Ophir also works with laser system developers to achieve embedded beam diagnostics to monitor system performance as an OEM solution.