InSight X3+ and InSight X3

InSight^®X3+^™ and InSight^®X3^™

WIDELY TUNABLE ULTRAFAST LASERS FOR MULTIPHOTON IMAGING

High power across entire tuning range with the industry’s highest power at
the multiphoton imaging wavelengths between 900 and 1300 nm
Field proven with the largest installed base in the market,
hundreds of peer-reviewed publications and demonstrated high reliability
Broad tuning range (680–1300 nm) for maximum flexibility
Dual output at 1045 nm with optional pre-compensation for simultaneous two color excitation

The InSight^® X3+^™ and InSight X3^™ are built on Spectra-Physics’ industry leading InSight platform designed for maximum performance and reliability. The InSight X3+ delivers 50% greater power across the tuning range for deeper imaging and more complex experiments. The InSight X3+ and InSight X3 lasers are built on the proven InSight platform with hundreds of peer-reviewed publications, the largest installed base in the market, and demonstrated high reliability. The InSight platform is specifically designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences.

Based on patented technology¹, InSight features a broad 680 nm to 1300 nm continuous, gap free, tuning curve from a single beam with nearly double the tuning range of legacy Ti:Sapphire lasers. The InSight is designed with the peak of the tuning curve centered in the 900 to 1100 nm region to excite the most common green and red fluorophores including GFP, RFP, GCaMP, jRGECO, and mCherry. The InSight X3+ additionally provides the highest powers at the longest wavelengths of 1280–1300 nm for excitation of far red fluorophores such as Alexa-680.

With Spectra-Physics’ integrated and patented DeepSee^™, the industry standard dispersion pre-compensation module, the short femtosecond pulses are optimally delivered through a microscope to the sample providing maximum fluorescence and penetration depth. The InSight has exceptional beam pointing stability, beam quality, output power stability and noise, with fast wavelength tuning.

When equipped with the fixed 1045 nm dual beam option, the InSight platform fully supports the diverse needs of multimodal imaging. The two synchronized output beams enable easy simultaneous imaging of various fluorescence proteins, genetically encoded calcium indicators, second and third harmonic generation, and advanced imaging techniques such as CARS and SRS. For these advanced techniques the InSight can be combined with the Newport Timing and Recombination Unit (TRU), or the Spectral Focusing Timing and Recombination Unit (SF-TRU).

1. Patents

Applications

Multiphoton microscopy

Two-Photon Excited Fluorescence (TPEF)

Multimodal imaging
-Coherent anti-stokes Raman Spectroscopy (CARS)
-Stimulated Raman spectroscopy (SRS)
-Second harmonic generation (SHG)
-Third harmonic generation (THG)

Optogenetics

Non-linear spectroscopy

Time-resolved photoluminescence

Optical computed tomography

Terahertz imaging

Semiconductor metrology

Output Characteristics^{1, 11}


	InSight X3+		InSight X3
	Main Output	Dual Output	Main Output	Dual Output
Tuning Range	680 nm–1300 nm	1045 nm (fixed)	680 nm–1300 nm	1045 nm (fixed)
Average Power²	>1.3 W at 700 nm >2.4 W at 800 nm >3.0 W at 900 nm >2.7 W at 1000 nm >2.4 W at 1100 nm >2.1 W at 1200 nm >1.5 W at 1300 nm	>3.5 W at 1045 nm	>1.0 W at 700 nm >1.6 W at 800 nm >2.0 W at 900 nm >1.8 W at 1000 nm >1.6 W at 1100 nm >1.4 W at 1200 nm >1.0 W at 1300 nm	>3.5 W at 1045 nm
Pulse Width^3,6	<120 fs	<170 fs	<120 fs	<200 fs
Noise^{3,4, 6}	<0.5%	<0.25%	<0.5%	<0.5%
Pre-compensation Dispersion Range²	680 nm: -12,000 fs² to -40,000 fs²800 nm: 0 fs² to -28,000 fs²900 nm: 0 fs² to -17,000 fs²1050 nm: 0 fs² to -13,000 fs² 1300 nm: -3,000 fs² to -11,000 fs²	"Optional 1045 nm: 15,000 fs² fixed"	680 nm: -12,000 fs² to -40,000 fs² 800 nm: 0 fs² to -25,000 fs² 1050 nm: 0 fs² to -10,000 fs² 1300 nm: -3,000 fs² to -8,000 fs²	"Optional 1045 nm: -15,000 fs² fixed"
Repetition Rate⁷	80 MHz ±0.5
Spatial Mode³	TEM₀₀, M² <1.2
Beam Diameter (1/e²)³	1.1 ±0.2 mm
Beam Divergence, full angle³	<1.5 mrad
Beam Roundness³	1.0 ±0.2 mm
Beam Astigmatism³³	<20 %
Beam Pointing Stability⁹	<350 µrad
Tuning Speed¹⁰	>50 nm/sec
Stability⁵	<±1 %
Polarization³	>500:1 horizontal

Environmental Requirements


	InSight X3+		InSight X3
	Main Output	Dual Output	Main Output	Dual Output
Altitude (m)	Up to 2000 M
Temperature, Operating	20–25°C
Relative Humidity, Operating	Maximum 75% non-condensing up to 25°C
Temperature, Storage	15–35°C
Relative Humidity, Storage	Maximum 65% for 15–35°C
Cooled Water Temperature in Closed-loop Chiller	21°C typical⁸

Electrical Requirements


	InSight X3+		InSight X3
	Main Output	Dual Output	Main Output	Dual Output
Power Supply	100–120 V~/200–240 V~, 50/60 Hz
Chiller	100–200 V~, 50/60 HzC
Total System Power Consumption	<1500 W
Communication Interface	RS-232, USB, PC required
Sync Signal	SMA

1. Due to our continuous improvement program, specifications may change without notice.
2. Specifications only apply to the wavelength noted.
3. Specification applies to 900 nm (tunable) or 1045 nm (fixed), respectively.
4. Specification represents rms noise measured in a 10 Hz to 10 MHz bandwidth.
5. Percent power drift in any 2-hour period with less than ±1°C temperature change after a 1-hour warm up.
6. A sech2 pulse shape is used to determine the pulse width as measured with a Newport PulseScout^® autocorrelator.
7. Tunable and fixed outputs are synchronized.
8. Avoid obstructing the air exhaust grills which will result in the recirculation of hot exhaust air.
Cooling air enters through the front panel and exits through the rear fan apertures.
9. Maximum deviation across the entire tuning range and pre-compensation dispersion range.
10. Averaged over entire tuning range.
11. InSight X3+ and InSight X3 are Class IV – High-Power Lasers, whose beams are, by definition, safety and fire hazards.
Take precautions to prevent exposure to direct and reflected beams. Diffuse as well as specular reflections can cause severe skin or eye damage.