• Flow Cytometry
  • Cell Sorting
  • Wafer Inspection
  • Electron Microscopy
  • Confocal Microscopy
  • Laser-induced Fluorescence


Output Characteristics1, 2

Vanguard One UV125
Wavelength 355 ± 1 nm
Power 125 mW
Repetition Rate 80 ± 2 MHz
Average Power Stability <2%
Pulse Energy Noise <1% rms
Beam Pointing Stability <25 µrad/°C
Polarization Ratio >100:1
Beam Quality (M2) <1.2 TEM00
Circularity 0.8-1.2
Beam Diameter (1/e2) 1 ± 0.2 mm
Beam Divergence <1 mrad
Laser Head (L x W x H) 313 x 259 x169 mm
Operating Temperature 20-27°C
Cooling Air
Heat Dissipation Maximum 160 W (typical 100 W)


  1. Due to our continuous product improvement program, specifications may change without notice.
  2. Vanguard One is a Class IV - High-Power Laser, whose beam is, by definition, a safety and fire hazard. Take precautions to prevent exposure to the direct and reflected beams. Diffuse as well as specular reflections can cause severe skin or eye damage.


Designed for 24/7 LHS Applications

The new laser operates air-cooled with low noise of <1% rms and delivers a long-operating life and low cost of ownership. Vanguard One is ideal for applications in bio-instrumentation, including flow cytometry, microscopy, cell sorting, and semiconductor wafer inspection.

OEM Integration

The quasi-CW operation ensures high fluorescence signal-to-noise ratio while preventing cell damage in bio-instrumentation. The laser firmware enables closed-loop power control, ensuring long-term stability with less than 2% power variation for consistent measurement results.

All-In-One Design

Based on the highly successful Vanguard laser family,Vanguard One UV125 is an all-in-one laser,combining electronics, laser cavity, and cooling in a single housing for easy integration and bench-top tools.


Vanguard One lasers pass extensive environmental qualification testing to deliver high reliability and low cost-of-ownership needed for bio-instrumentation and demanding industrial applications.

Compatible Power Sensors

To verify and analyze the output power of your Vanguard One laser, we recommend the Ophir 3A (7Z02621) thermal power sensor and Centauri laser power meter.  Additional information on this laser measurement equipment can be found on the ophiropt.com website.  Other Ophir sensors and meters may also be suitable for the Vanguard One depending on application. Ophir also works with laser system developers to achieve embedded beam diagnostics to monitor system performance as an OEM solution.