Widely Tunable Femtosecond Lasers for Multiphoton Imaging
- Field proven with the largest installed base in the market
- High reliability with >1,000 peer-reviewed publications
- Broad tuning range (680–1300 nm) for maximum flexibility
- Highest peak power for maximum fluorescence
- InSight X3+ A and InSight X3 A models with integrated attenuation 모든 기능 보기
Applications
사양
Characteristics1, 8
| InSight X3+ | InSight X3 | ||||
|---|---|---|---|---|---|
| Output Characteristics | Main Output | Dual Output | Main Output | Dual Output | |
| Tuning Range | 680 nm-1300 nm | 1045 nm (fixed) | 680 nm-1300 nm | 1045 nm (fixed) | |
| Average Power2 | >1.5 W at 700 nm >2.4 W at 800 nm >3.0 W at 900 nm >2.7 W at 1000 nm >2.4 W at 1100 nm >2.1 W at 1200 nm >1.5 W at 1300 nm | >3.5 W | >1.0 W at 700 nm >1.6 W at 800 nm >2.0 W at 900 nm >1.8 W at 1000 nm >1.6 W at 1100 nm >1.4 W at 1200 nm >1.0 W at 1300 nm | >3.5 W | |
| Pulse Width2, 3 | <120 fs | <170 fs | <120 fs | <200 fs | |
| Noise2, 4 | <0.5 % | <0.25 % | <0.5 % | <0.5 % | |
| Pre-compensation Dispersion Range | 680 nm: -12,000 fs2 to -40,000 fs2 800 nm: 0 fs2 to -25,000 fs2 1050 nm: 0 fs2 to -10,000 fs2 1300 nm: -3,000 fs2 to -8,000 fs2 | "Optional: -14,000 fs2 fixed" | 680 nm: -12,000 fs2 to -40,000 fs2 800 nm: 0 fs2 to -25,000 fs2 1050 nm: 0 fs2 to -10,000 fs2 1300 nm: -3,000 fs2 to -8,000 fs2 | "Optional: -14,000 fs2 fixed" | |
| Repetition Rate | 80 MHz ±0.5 | ||||
| Spatial Mode2 | TEM00, M2 <1.2 | ||||
| Beam Diameter (1/e²)2 | 1.1 ±0.2 mm | ||||
| Beam Divergence, full angle2 | <1.5 mrad | ||||
| Beam Roundness2 | 0.8-1.2 | ||||
| Beam Astigmatism2 | <20 % | ||||
| Beam Pointing Stability5 | <350 µrad | ||||
| Tuning Speed6 | >50 nm/sec | ||||
| Stability7 | <±1 % | ||||
| Polarization2 | >500:1 horizontal | ||||
Environmental Requirements
| InSight X3+ | InSight X3 | ||||
|---|---|---|---|---|---|
| Altitude | Up to 2000 m | ||||
| Temperature, Operating | 20-25°C | ||||
| Relative Humidity, Operating | Maximum 75% non-condensing up to 25°C | ||||
| Temperature, Storage | 15-35°C | ||||
| Relative Humidity, Storage | Maximum 65% for 15-35°C | ||||
| Cooled Water Temperature in Closed-loop Chiller | 21°C, Typical | ||||
Electrical Requirements
| InSight X3+ | InSight X3 | ||||
|---|---|---|---|---|---|
| Power Supply | 100-120 V~/200-240 V~, 50/60 Hz | ||||
| Chiller | 100-200 V~, 50/60 HzC | ||||
| Total System Power Consumption | <1500 W | ||||
| Communication Interface | RS-232, USB, PC required | ||||
| Sync Signal | SMA | ||||
Footnotes:
- Due to our continuous improvement program, specifications may change without notice.
- Specification applies to 900 nm (tunable) output.
- A sech2 pulse shape is used to determine the pulse width as measured with a Newport PulseScout® autocorrelator.
- Specification represents rms noise measured in a 10 Hz to 10 MHz bandwidth.
- Maximum deviation across the entire tuning range and precompensation dispersion range.
- Averaged over entire tuning range.
- Percent power drift in any 2-hour period with less than ±1°C temperature change after a 1-hour warm up.
- InSight X3+ and InSight X3 are Class IV – High- Power Lasers, whose beams are, by definition, safety and fire hazards. Take precautions to prevent exposure to direct and reflected beams. Diffuse as well as specular reflections can cause severe skin or eye damage.
- TypeFemtosecond Laser
- Power1000-2000 mW (ave.)
- Wavelength RegionTunable
- 펄스폭fs
- Wavelength680-1300 nm
- ApplicationIndustrial Manufacturing
- Process TypeMetrology
- ProcessTwo-photon Polymerization
기능
Overview
The InSight X3+ and InSight X3 are built on Spectra-Physics’ industry leading InSight platform. Designed for maximum performance and reliability, the InSight X3+ delivers high powers across the tuning range for brighter signals and increased imaging depths. The InSight X3+ and InSight X3 lasers have the largest
installed base in the market. As a result, researchers have generated more than 1,000 peer-reviewed
publications.
Ultra-Wide Tuning Range
The InSight platform is designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences. Based on patented technology1, InSight is a hands-free, fully computer-controlled light source. It features a gapfree, 680 nm to 1300 nm tuning range which nearly doubles the accessible wavelengths when compared to traditional Ti:sapphire lasers. The InSight performance is optimized in the 900–1100 nm range to support the common green and red fluorescent proteins including GFP, RFP, GCaMP, jRGECO, and mCherry. With the InSight X3+ A increased output power, experiments using red flurophores in the 1200–1300 nm range can be performed.
Exceptional Performance
InSight X3+ A and InSight X3 A are equipped with the standard, DeepSee integrated dispersion compensation to ensure pulse broadening effects are minimized. As a result, fluorescence signal and penetration depth are maximized. The integrated attenuators provide real-time adjustment of the output energy delivered to the sample. Their fast response time acts as a software-controlled switch that can be used to minimize exposure for delicate samples and to minimize backlash effects.
Both lasers can be equipped with a fixed 1045 nm dual beam option to support the diverse needs of multimodal imaging. The tunable and fixed outputs are synchronized which enables several applications including advanced imaging techniques such as CARS and SRS. The InSight also supports simultaneous, multi-wavelength excitation of fluorophores and/or genetically encoded calcium indicators, and second and third harmonic generation
microscopies.
호환 가능한 파워 센서
InSight X3+/X3 레이저의 출력 확인과 분석은, Ophir 10A-BB-16 열 전력 센서와 Centauri 레이저 파워 미터를 추천합니다. 이 레이저 측정 장비에 대한 추가 정보는 ophiropt.com 에서 웹사이틍서 찾아 볼 수 있습니다. 다른 Ophir 센서와 미터기도 용도에 따라 InSight X3+/X3 에 적합할 수 있습니다. 또한 Ophir 는 레이저 시스템 개발자들과의 협력을 통해 임베디드 빔 진단법을 구축하여, OEM 솔루션으로서 시스템 성능을 모니터합니다.
InSight X3+ A and InSight X3 A Models
Built on the proven InSight platform, InSight X3+ A and InSight X3 A lasers are equipped with integrated attenuation for real-time optimization of the experimental pulse energy. Each model delivers high average power, short pulses and dispersion compensation to support the most demanding experiments in neuroscience, immunology and biology.
리소스
Technical Articles
Multiphoton Microscopy Provides a Deeper View of the Aging Brain(2 MB, PDF) 뇌졸증의 충격을 포착하는 NIR 분광법(2.4 MB, PDF) DPSS UV Lasers Improve Mass Spectrometry and Laser Microdissection.pdf(705.6 kB, PDF) 다광자 현미경: 다광자 이미징의 성장을 촉진하는 턴키 펨토초 레이저(178.7 kB, PDF) 2광자 광유전학을 발전시키는 펨토초 레이저 다광자 이미징/신경과학: 초고속 레이저로 신경 과학을 더 깊이 있게 신경 활동 이미징을 위한 민감형 적색 단백질 칼슘 표시자 고도로 산란된 광자로부터 자극된 라만 신호를 검색하는 분광계가 필요 없는 진동 이미징 바이오이미징을 발전시키는 초고도 피크 파워 펨토초 레이저(823.8 kB, PDF) 펨토초 레이저: 더 많은 미세 가공 어플리케이션 높은 피크 출력의 1042 nm 레이저를 활용한 다점 스캐닝 2광자 여기 현미경 다광자 이미징을 위한 소형 고정 파장 펨토초 발진기 새로운 방향을 제시하는 다광자 이미징(1.1 MB, PDF) 고친화성, 고속, 적색 칼슘 지시약 R-CAMP2의 합리적인 설계 다광자 이미징 심층 분석(717.1 kB, PDF) 고정 파장 펨토초 레이저를 이용한 2광자 현미경(801.5 kB, PDF) 2 광자 현미경/다중 모드 이미징: 펨토초 레이저 개발로 2 광자 이미징의 향상(459.8 kB, PDF) 다광자 이미징의 새로운 차원(2.4 MB, PDF) M2 가 중요한 이유
Drawings & CAD
InSight X3+/X3 and InSight X3+/X3 A Dimensions(263.7 kB, PDF)
사전 설치 가이드
InSight X3+ 그리고 InSight X3사전 설치 가이드(473.6 kB, PDF)
Literature
Ultrafast Lasers Brochure(4.6 MB, PDF)
Application Notes
Data Sheets
InSight X3+ and InSight X3 Datasheet(856.7 kB, PDF) InSight X3+ A and InSight X3 A Datasheet(844.7 kB, PDF)


