受激拉曼光谱

在SRS 中,泵浦光和斯托克斯光都入射 到样品上。如果频率差与样品的分子振动相匹配,就会激发振动跃迁。基于SRS 的显微技术是一种相对较新的技 先进的生物成像 术,已在2008 年被证明,并在最近几年实现了商品化。
Energy level diagram for the SGH mechanism
Figure 1. Energy level diagram for the SRS imaging mechanism.

Stimulated Raman Scattering Bio-Imaging Examples

SRS image of fatty liver, pump 802 nm and Stokes at 1040 nm.
Figure 2. SRS image of fatty liver, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Ji-XIn Cheng, Purdue University

SRS image of spinal cord, pump 802 nm and Stokes at 1040 nm
Figure 3. SRS image of spinal cord, pump 802 nm and Stokes at 1040 nm; acquired with InSight DS+.
Courtesy of Dr. Marc van Zandvoort, Maastrich University