The Spirit-NOPA®-3H, pumped with a Spirit® 1030-70/100 industrial femtosecond laser, is our leading platform for 500-900 nm three-photon (3P) imaging, enabling breakthrough applications in neuroscience. 3P excitation fluorescence microscopy allows imaging at unprecedented resolution and tissue depth. Utilizing the unique capability of this imaging source for 3P, very high acquisition rates are possible. While 3P requires higher peak powers compared to two-photon (2P) excitation, it yields better optical confinement and signal-to-background ratio in deep tissue imaging, leading to improved image resolution and contrast. Spectra-Physics’ 3P imaging platform enables fast image acquisition due to the very high repetition rates which are available from the source.